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peripheral blood cd14 monocytes pbmc  (ATCC)


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    ATCC peripheral blood cd14 monocytes pbmc
    Peripheral Blood Cd14 Monocytes Pbmc, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/peripheral+blood+cd14+monocytes+pbmc/Primary+Peripheral+Blood+CD14%2B+Monocytes%2C+Normal%2C+Human/pmc12726827-141-2-10
    Average 99 stars, based on 66 article reviews
    peripheral blood cd14 monocytes pbmc - by Bioz Stars, 2026-09
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    other:

    Article Title: Trained Immunity: RoadMap for drug discovery and development
    Article Snippet: Primary , Peripheral Blood CD14+ Monocytes (PBMC) , Human , ATCC Product Code: ATCC-PCS-800–010 , https://www.lgcstandards.com/GB/en/search?text=monocyte%20cell%20lines.



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    Cell culture supernatant from miR-1260b mimic/ATF6β small interfering RNA (siRNA)-transfected PDL cells inhibits osteoclastogenesis in CD14 + peripheral blood-derived monocytes (PBMCs). (A) Scheme for the validation of the effect of cell culture supernatant from PDL cells on osteoclast differentiation. PDL cells were transfected with either miRNA mimic (miR-Ctrl or miR-1260b) or siRNA (si-Ctrl or si-ATF6β) for 24 h. After changing the medium, cells were stimulated with tunicamycin (0.5 μg/ml) for 24 h. CD14 + PBMCs were stimulated with M-CSF for 3 days, and then incubated with the cell culture supernatant from PDL cells as described above for additional 3 days. PBMCs were further stimulated with the receptor activator of NF-κB ligand (RANKL)/macrophage colony-stimulating factor (M-CSF) for up to 14 days (B) Representative images of tartrate-resistant acid phosphatase (TRAP) staining. Scale bar = 200 μm. (C,D) Osteoclast differentiation was evaluated by the number of TRAP-positive multinucleated cells (MNCs) (7 or 10 ≤ nuclei) (C) and the size of TRAP-positive MNCs (3 ≤ nuclei) (D) . n = 3. (E) Representative images of the resorption pit on the calcium phosphate (CaP)-coated plate. Scale bar = 200 μm. (F) Total resorbed area in each culture was measured. n = 3. * p < 0.05, ** p < 0.01, **** p < 0.0001. The significance of differences between groups was determined using one-way ANOVA, followed by correction for multiple comparisons with Tukey’s post hoc test.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: miR-1260b inhibits periodontal bone loss by targeting ATF6β mediated regulation of ER stress

    doi: 10.3389/fcell.2022.1061216

    Figure Lengend Snippet: Cell culture supernatant from miR-1260b mimic/ATF6β small interfering RNA (siRNA)-transfected PDL cells inhibits osteoclastogenesis in CD14 + peripheral blood-derived monocytes (PBMCs). (A) Scheme for the validation of the effect of cell culture supernatant from PDL cells on osteoclast differentiation. PDL cells were transfected with either miRNA mimic (miR-Ctrl or miR-1260b) or siRNA (si-Ctrl or si-ATF6β) for 24 h. After changing the medium, cells were stimulated with tunicamycin (0.5 μg/ml) for 24 h. CD14 + PBMCs were stimulated with M-CSF for 3 days, and then incubated with the cell culture supernatant from PDL cells as described above for additional 3 days. PBMCs were further stimulated with the receptor activator of NF-κB ligand (RANKL)/macrophage colony-stimulating factor (M-CSF) for up to 14 days (B) Representative images of tartrate-resistant acid phosphatase (TRAP) staining. Scale bar = 200 μm. (C,D) Osteoclast differentiation was evaluated by the number of TRAP-positive multinucleated cells (MNCs) (7 or 10 ≤ nuclei) (C) and the size of TRAP-positive MNCs (3 ≤ nuclei) (D) . n = 3. (E) Representative images of the resorption pit on the calcium phosphate (CaP)-coated plate. Scale bar = 200 μm. (F) Total resorbed area in each culture was measured. n = 3. * p < 0.05, ** p < 0.01, **** p < 0.0001. The significance of differences between groups was determined using one-way ANOVA, followed by correction for multiple comparisons with Tukey’s post hoc test.

    Article Snippet: Human CD14 + peripheral blood-derived monocytes (PBMCs) were purchased from Lonza (Basel, Switzerland).

    Techniques: Cell Culture, Small Interfering RNA, Transfection, Derivative Assay, Incubation, Staining